cfx96 touchtm real time fluorescent quantitative pcr instrument (Bio-Rad)
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cfx96 touchtm real time fluorescent quantitative pcr instrument
Cfx96 Touchtm Real Time Fluorescent Quantitative Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 28077 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+quantitative+pcr+instrument/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41900994-158-6-13
Average 96 stars, based on 28077 article reviews
Cfx96 Touchtm Real Time Fluorescent Quantitative Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 28077 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+quantitative+pcr+instrument/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41900994-158-6-13
Average 96 stars, based on 28077 article reviews
cfx96 touchtm real time fluorescent quantitative pcr instrument - by Bioz Stars,
2026-09
96/100 stars
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Expressing:Article Title: Geniposide reduced oxidative stress-induced apoptosis in HK-2 cell through PI3K/AKT3/FOXO1 by m6A modification. Article Snippet: Exogenous hydrogen peroxide (H2O2) may generate excessive oxidative stress, inducing renal cell apoptosis related with kidney dysfunction.. Geniposide (GP) belongs to the iridoid compound with anti-inflammatory, antioxidant and anti-apoptotic effects.. This study aimed to observe the intervention effect of GP on H2O2induced apoptosis in human kidney-2 (HK-2) cells and to explore its potential mechanism in relation to N6methyladenosine (m6A) RNA methylation. Real-time Polymerase Chain Reaction:Article Title: Geniposide reduced oxidative stress-induced apoptosis in HK-2 cell through PI3K/AKT3/FOXO1 by m6A modification. Article Snippet: Exogenous hydrogen peroxide (H2O2) may generate excessive oxidative stress, inducing renal cell apoptosis related with kidney dysfunction.. Geniposide (GP) belongs to the iridoid compound with anti-inflammatory, antioxidant and anti-apoptotic effects.. This study aimed to observe the intervention effect of GP on H2O2induced apoptosis in human kidney-2 (HK-2) cells and to explore its potential mechanism in relation to N6methyladenosine (m6A) RNA methylation. Article Title: Treatment of cholangiocarcinoma by pGCsiRNA-vascular endothelial growth factor in vivo Article Snippet: Total RNA was extracted with 1 mL of TRIzol (Invitrogen, USA) and its concentration was measured. .. Then, 500 ng of total RNA was reversely transcribed into cDNA according to the instructions of the PrimeScript RT kit (Takara, Japan), and PCR was performed on a Article Title: HOXD10 regulates intestinal permeability and inhibits inflammation of dextran sulfate sodium-induced ulcerative colitis through the inactivation of the Rho/ROCK/MMPs axis Article Snippet: A BeyoRTTM First Strand cDNA Synthesis Kit (D7166, Beyotime, China) was utilized to produce the complementary DNA according to the protocol of the kit. .. Then the qRT-PCR was conducted with MonAmpTM Taqman qPCR Mix (MQ30101S, MQ30101S, Monad, China) on a Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway. Article Snippet: 249 Electric blast dryer (Shanghai Experimental Instrument Factory Co., Ltd.), slicer (Leica 250 2015, Germany), TSJ-Q fully automatic closed tissue dehydrator, BMJ-III embedding 251 machine, PHY-III pathological tissue bleaching and drying instrument (Changzhou 252 Zhongwei Medical Instrument Co., Ltd.), Motic BA400 microscope, inverted 253 microscope (Olympus, Japan) and TEK-II MINI fully automatic three-group blood 254 analyser (Jiangxi Tekang Technology Co., Ltd.) were used. .. 255 The following equipment were used: CO2 incubator (Thermo, USA), DW-86L386 256 vertical ultralow-temperature storage box (Qingdao Haier Special Electrical 257 Appliances Co., Ltd.), H-2050R desktop high-speed freezing centrifuge, LDZX-3-KB 258 Jo urn al Pr e-p r f 11 vertical pressure steam sterilisation pot (Shanghai Shen’an Medical Device Factory), 259 Varioskan flash full-wavelength scanning multifunctional reader (Thermo, USA), UV-260 1800 spectrophotometer (Shanghai Meipeda Instrument Co., Ltd.), Article Title: Rac3 promotes proliferation and invasion of endometrial cancer through the AKT/mTOR signalling pathway. Article Snippet: They were cultured in an incubator with 5% co2 at 37 °c, using RPMi-1640 medium enriched with 10% foetal bovine serum (FBS) and 1% antibiotics. qRT-PCR assay Total Rna was extracted from tissues or cells using Trizol reagent (R0016, Beyotime). after determining the concentration and purity, the Rna was reverse transcribed into cDna. .. Subsequently, following the instructions of the SyBR® Green Quantitative RT-qPcR Kit (QR0100, Sigma-aldrich), and using a Article Title: Morphological, physiological, and transcriptomic analyses indicate that cell wall properties and antioxidant processes are potential targets for improving the aluminium tolerance of broad beans. Article Snippet: Aluminium (Al) stress is the second-leading abiotic stress on crops.. An improved understanding of the response mechanisms of plants to Al stress will provide scientific guidance for enhancing the crops’ tolerance to Al stress.. 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Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Fluorescence:Article Title: Geniposide reduced oxidative stress-induced apoptosis in HK-2 cell through PI3K/AKT3/FOXO1 by m6A modification. Article Snippet: Exogenous hydrogen peroxide (H2O2) may generate excessive oxidative stress, inducing renal cell apoptosis related with kidney dysfunction.. Geniposide (GP) belongs to the iridoid compound with anti-inflammatory, antioxidant and anti-apoptotic effects.. This study aimed to observe the intervention effect of GP on H2O2induced apoptosis in human kidney-2 (HK-2) cells and to explore its potential mechanism in relation to N6methyladenosine (m6A) RNA methylation. Article Title: Treatment of cholangiocarcinoma by pGCsiRNA-vascular endothelial growth factor in vivo Article Snippet: Total RNA was extracted with 1 mL of TRIzol (Invitrogen, USA) and its concentration was measured. .. Then, 500 ng of total RNA was reversely transcribed into cDNA according to the instructions of the PrimeScript RT kit (Takara, Japan), and PCR was performed on a Article Title: HOXD10 regulates intestinal permeability and inhibits inflammation of dextran sulfate sodium-induced ulcerative colitis through the inactivation of the Rho/ROCK/MMPs axis Article Snippet: A BeyoRTTM First Strand cDNA Synthesis Kit (D7166, Beyotime, China) was utilized to produce the complementary DNA according to the protocol of the kit. .. Then the qRT-PCR was conducted with MonAmpTM Taqman qPCR Mix (MQ30101S, MQ30101S, Monad, China) on a Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway. Article Snippet: 249 Electric blast dryer (Shanghai Experimental Instrument Factory Co., Ltd.), slicer (Leica 250 2015, Germany), TSJ-Q fully automatic closed tissue dehydrator, BMJ-III embedding 251 machine, PHY-III pathological tissue bleaching and drying instrument (Changzhou 252 Zhongwei Medical Instrument Co., Ltd.), Motic BA400 microscope, inverted 253 microscope (Olympus, Japan) and TEK-II MINI fully automatic three-group blood 254 analyser (Jiangxi Tekang Technology Co., Ltd.) were used. .. 255 The following equipment were used: CO2 incubator (Thermo, USA), DW-86L386 256 vertical ultralow-temperature storage box (Qingdao Haier Special Electrical 257 Appliances Co., Ltd.), H-2050R desktop high-speed freezing centrifuge, LDZX-3-KB 258 Jo urn al Pr e-p r f 11 vertical pressure steam sterilisation pot (Shanghai Shen’an Medical Device Factory), 259 Varioskan flash full-wavelength scanning multifunctional reader (Thermo, USA), UV-260 1800 spectrophotometer (Shanghai Meipeda Instrument Co., Ltd.), Article Title: Rac3 promotes proliferation and invasion of endometrial cancer through the AKT/mTOR signalling pathway. 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In this study, Al stress (50–200 μM AlCl3) caused visible damage to broad bean (Vicia faba L.) roots rather than shoots, which was attributed to Al accumulation and distribution in different tissues. Article Title: Synbiotics containing sea buckthorn polysaccharides ameliorate DSS-induced colitis in mice via regulating Th17/Treg homeostasis through intestinal microbiota and their production of BA metabolites and SCFAs. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Polymerase Chain Reaction:Article Title: Treatment of cholangiocarcinoma by pGCsiRNA-vascular endothelial growth factor in vivo Article Snippet: Total RNA was extracted with 1 mL of TRIzol (Invitrogen, USA) and its concentration was measured. .. Then, 500 ng of total RNA was reversely transcribed into cDNA according to the instructions of the PrimeScript RT kit (Takara, Japan), and PCR was performed on a Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway. Article Snippet: 249 Electric blast dryer (Shanghai Experimental Instrument Factory Co., Ltd.), slicer (Leica 250 2015, Germany), TSJ-Q fully automatic closed tissue dehydrator, BMJ-III embedding 251 machine, PHY-III pathological tissue bleaching and drying instrument (Changzhou 252 Zhongwei Medical Instrument Co., Ltd.), Motic BA400 microscope, inverted 253 microscope (Olympus, Japan) and TEK-II MINI fully automatic three-group blood 254 analyser (Jiangxi Tekang Technology Co., Ltd.) were used. .. 255 The following equipment were used: CO2 incubator (Thermo, USA), DW-86L386 256 vertical ultralow-temperature storage box (Qingdao Haier Special Electrical 257 Appliances Co., Ltd.), H-2050R desktop high-speed freezing centrifuge, LDZX-3-KB 258 Jo urn al Pr e-p r f 11 vertical pressure steam sterilisation pot (Shanghai Shen’an Medical Device Factory), 259 Varioskan flash full-wavelength scanning multifunctional reader (Thermo, USA), UV-260 1800 spectrophotometer (Shanghai Meipeda Instrument Co., Ltd.), Quantitative RT-PCR:Article Title: Treatment of cholangiocarcinoma by pGCsiRNA-vascular endothelial growth factor in vivo Article Snippet: Total RNA was extracted with 1 mL of TRIzol (Invitrogen, USA) and its concentration was measured. .. Then, 500 ng of total RNA was reversely transcribed into cDNA according to the instructions of the PrimeScript RT kit (Takara, Japan), and PCR was performed on a Article Title: HOXD10 regulates intestinal permeability and inhibits inflammation of dextran sulfate sodium-induced ulcerative colitis through the inactivation of the Rho/ROCK/MMPs axis Article Snippet: A BeyoRTTM First Strand cDNA Synthesis Kit (D7166, Beyotime, China) was utilized to produce the complementary DNA according to the protocol of the kit. .. Then the qRT-PCR was conducted with MonAmpTM Taqman qPCR Mix (MQ30101S, MQ30101S, Monad, China) on a Serial Time-encoded Amplified Microscopy:Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway. Article Snippet: 249 Electric blast dryer (Shanghai Experimental Instrument Factory Co., Ltd.), slicer (Leica 250 2015, Germany), TSJ-Q fully automatic closed tissue dehydrator, BMJ-III embedding 251 machine, PHY-III pathological tissue bleaching and drying instrument (Changzhou 252 Zhongwei Medical Instrument Co., Ltd.), Motic BA400 microscope, inverted 253 microscope (Olympus, Japan) and TEK-II MINI fully automatic three-group blood 254 analyser (Jiangxi Tekang Technology Co., Ltd.) were used. .. 255 The following equipment were used: CO2 incubator (Thermo, USA), DW-86L386 256 vertical ultralow-temperature storage box (Qingdao Haier Special Electrical 257 Appliances Co., Ltd.), H-2050R desktop high-speed freezing centrifuge, LDZX-3-KB 258 Jo urn al Pr e-p r f 11 vertical pressure steam sterilisation pot (Shanghai Shen’an Medical Device Factory), 259 Varioskan flash full-wavelength scanning multifunctional reader (Thermo, USA), UV-260 1800 spectrophotometer (Shanghai Meipeda Instrument Co., Ltd.), Spectrophotometry:Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway. Article Snippet: 249 Electric blast dryer (Shanghai Experimental Instrument Factory Co., Ltd.), slicer (Leica 250 2015, Germany), TSJ-Q fully automatic closed tissue dehydrator, BMJ-III embedding 251 machine, PHY-III pathological tissue bleaching and drying instrument (Changzhou 252 Zhongwei Medical Instrument Co., Ltd.), Motic BA400 microscope, inverted 253 microscope (Olympus, Japan) and TEK-II MINI fully automatic three-group blood 254 analyser (Jiangxi Tekang Technology Co., Ltd.) were used. .. 255 The following equipment were used: CO2 incubator (Thermo, USA), DW-86L386 256 vertical ultralow-temperature storage box (Qingdao Haier Special Electrical 257 Appliances Co., Ltd.), H-2050R desktop high-speed freezing centrifuge, LDZX-3-KB 258 Jo urn al Pr e-p r f 11 vertical pressure steam sterilisation pot (Shanghai Shen’an Medical Device Factory), 259 Varioskan flash full-wavelength scanning multifunctional reader (Thermo, USA), UV-260 1800 spectrophotometer (Shanghai Meipeda Instrument Co., Ltd.), Electrophoresis:Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway. Article Snippet: 249 Electric blast dryer (Shanghai Experimental Instrument Factory Co., Ltd.), slicer (Leica 250 2015, Germany), TSJ-Q fully automatic closed tissue dehydrator, BMJ-III embedding 251 machine, PHY-III pathological tissue bleaching and drying instrument (Changzhou 252 Zhongwei Medical Instrument Co., Ltd.), Motic BA400 microscope, inverted 253 microscope (Olympus, Japan) and TEK-II MINI fully automatic three-group blood 254 analyser (Jiangxi Tekang Technology Co., Ltd.) were used. .. 255 The following equipment were used: CO2 incubator (Thermo, USA), DW-86L386 256 vertical ultralow-temperature storage box (Qingdao Haier Special Electrical 257 Appliances Co., Ltd.), H-2050R desktop high-speed freezing centrifuge, LDZX-3-KB 258 Jo urn al Pr e-p r f 11 vertical pressure steam sterilisation pot (Shanghai Shen’an Medical Device Factory), 259 Varioskan flash full-wavelength scanning multifunctional reader (Thermo, USA), UV-260 1800 spectrophotometer (Shanghai Meipeda Instrument Co., Ltd.), SYBR Green Assay:Article Title: Rac3 promotes proliferation and invasion of endometrial cancer through the AKT/mTOR signalling pathway. Article Snippet: They were cultured in an incubator with 5% co2 at 37 °c, using RPMi-1640 medium enriched with 10% foetal bovine serum (FBS) and 1% antibiotics. qRT-PCR assay Total Rna was extracted from tissues or cells using Trizol reagent (R0016, Beyotime). after determining the concentration and purity, the Rna was reverse transcribed into cDna. .. Subsequently, following the instructions of the SyBR® Green Quantitative RT-qPcR Kit (QR0100, Sigma-aldrich), and using a other:Article Title: First Clarification of the Mechanism of Action of the Apple Glycosyltransferase MdUGT91AJ2 Involved in the Detoxification Metabolism of the Triketone Herbicide Sulcotrione. Article Snippet: Re -time PCR: The UltraSYBR Mixture kit (CW0659S, ang ei entury Technology Co., Ltd., Beijing, China) was used, and th fluorescence qu titative PCR instrument model was the |
